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EWS-WT1 translocation by RT PCR
The EWS-WT1 translocation by RT PCR test is used to detect a specific gene fusion between the EWSR1 gene on chromosome 22 and the WT1 gene on chromosome 11, resulting from the t(11;22)(p13;q12) translocation. This fusion is a hallmark of desmoplastic small rou...
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The EWS-WT1 translocation by RT PCR test is used to detect a specific gene fusion between the EWSR1 gene on chromosome 22 and the WT1 gene on chromosome 11, resulting from the t(11;22)(p13;q12) translocation. This fusion is a hallmark of desmoplastic...
Understanding EWS-WT1 translocation by RT PCR
The EWS-WT1 translocation by RT PCR test is used to detect a specific gene fusion between the EWSR1 gene on chromosome 22 and the WT1 gene on chromosome 11, resulting from the t(11;22)(p13;q12) translocation. This fusion is a hallmark of desmoplastic small round cell tumor (DSRCT), a rare and aggressive type of soft tissue sarcoma. In this test, RNA is extracted from the sample and converted to cDNA, which is then evaluated for quality using a housekeeping gene assay (PGK assay). A positive result confirms that the...
What does EWS-WT1 translocation by RT PCR measure?
The EWS-WT1 translocation by RT PCR test is used to detect a specific gene fusion between the EWSR1 gene on chromosome 22 and the WT1 gene on chromosome 11, resulting from the t(11;22)(p13;q12) translocation. This fusion is a hallmark of desmoplastic small round cell tumor (DSRCT), a rare and aggressive type of soft tissue sarcoma. In this test, RNA is extracted from the sample and converted to cDNA, which is then evaluated for quality using a housekeeping gene assay (PGK assay). A positive result confirms that the...
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